Methylene blue stains nuclei and is a common teaching stain. Wear gloves – it dyes skin and fabric. Filter if particles remain. Dilute further for working stain as your protocol requires.
Steps
- Dissolve 1 g methylene blue powder in ~80 ml demineralised water.
- Top up to 100 ml; mix until dissolved; filter if needed.
- Label as stock; dilute to working strength before use on slides.
Materials required
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Methylene Blue Powder
1 g
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Demineralised Water
to 100 ml
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Always read the SDS and follow our chemical safety guidance before handling any chemical.